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Continuous Micro-Production Using Enzymatic Reaction and Online Monitoring
| Kontakt | Simon Crelier |
| Titel | Continuous Micro-Production Using Enzymatic Reaction and Online Monitoring |
| Autoren | Crelier S., Moser R., Chappuis Th., Vanoli E., Naef O. |
| Referenzen | Chimia (2010), 64, 799-802 |
| Abstract | A micro-reactor coupled to a microfluidic system and an online UV/VIS spectrometer is described. The enzymatic reaction studied is the hydrolysis of the N-benzoyl-L-tyrosine ethyl ester (BTEE) to N-benzoyl-L-tyrosine (BT) and ethanol, catalyzed by chymotrypsin. The production is online monitored with UV spectroscopy at 256 nm. Three different immobilization methods of the enzyme are discussed: Eupergit C, controlled-pore glass (CPG), and Sepharose. |
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